Journal: Biomedical Journal

Article Title: Inhibition of infantile hemangioma growth and promotion of apoptosis via VEGF/PI3K/Akt axis by 755-nm long-pulse alexandrite laser

doi: 10.1016/j.bj.2023.100675

Figure Lengend Snippet: The sequences of primers.

Article Snippet: The membranes were gently agitated and incubated with antibodies against the following proteins: VEGFA (BA0407, Boster, Wuhan, China, 1 : 1000), VEGFR2 (A00901-2, Boster, Wuhan, China, 1 : 1000), p-Akt (#4691, Cell Signaling Technology, MA, United States, 1 : 1000), p-Akt (#4060, Cell Signaling Technology, MA, United States, 1 : 2000), VEGF 165a (ab69479, Abcam, Cambridge, MA, United States), VEGF 165b (GTX52502, GeneTex, Irvine, CA, United States), glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (A00227-4, Boster, Wuhan, China, 1 : 1000), at 4 °C overnight.

Techniques:

Journal: Biomedical Journal

Article Title: Inhibition of infantile hemangioma growth and promotion of apoptosis via VEGF/PI3K/Akt axis by 755-nm long-pulse alexandrite laser

doi: 10.1016/j.bj.2023.100675

Figure Lengend Snippet: The downregulation of VEGFA expression and suppression of the PI3K/AKT signaling pathway in HemECs by long-pulsed Alexandrite laser (755 nm). (A) Expression analysis of VEGFA, VEGFR2, PI3K, and Akt using quantitative RT-PCR. (B) Western blot analysis of the relative protein expression levels of VEGFA, VEGFR2, p-Akt, and Akt in HemECs. ∗∗ p <0.01, ∗∗∗ p <0.001 vs. control. Abbreviations: HemECs: human hemangioma endothelial cells; VEGF: vascular endothelial growth factor; VEGFR: vascular endothelial growth factor receptor; PI3K: phosphoinositide 3-kinase; Akt: protein kinase B.

Article Snippet: The membranes were gently agitated and incubated with antibodies against the following proteins: VEGFA (BA0407, Boster, Wuhan, China, 1 : 1000), VEGFR2 (A00901-2, Boster, Wuhan, China, 1 : 1000), p-Akt (#4691, Cell Signaling Technology, MA, United States, 1 : 1000), p-Akt (#4060, Cell Signaling Technology, MA, United States, 1 : 2000), VEGF 165a (ab69479, Abcam, Cambridge, MA, United States), VEGF 165b (GTX52502, GeneTex, Irvine, CA, United States), glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (A00227-4, Boster, Wuhan, China, 1 : 1000), at 4 °C overnight.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control

Journal: Biomedical Journal

Article Title: Inhibition of infantile hemangioma growth and promotion of apoptosis via VEGF/PI3K/Akt axis by 755-nm long-pulse alexandrite laser

doi: 10.1016/j.bj.2023.100675

Figure Lengend Snippet: The downregulation of VEGFA expression and suppression of PI3K/AKT signaling pathway activation in xenograft by long-pulsed Alexandrite laser (755 nm). (A) Analysis of the expression levels of VEGFA, VEGFR2, PI3K, and Akt using quantitative RT-PCR. (B) Western blot analysis of the protein expression levels of VEGFA, VEGFR2, p-Akt, and Akt in xenograft. (C) Detection of the relative protein expression levels of VEGFA 165a and VEGFA 165b in xenograft using western blot. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001 vs. control. Abbreviations: VEGF: vascular endothelial growth factor; VEGFR: vascular endothelial growth factor receptor; PI3K: phosphoinositide 3-kinase; Akt: protein kinase B.

Article Snippet: The membranes were gently agitated and incubated with antibodies against the following proteins: VEGFA (BA0407, Boster, Wuhan, China, 1 : 1000), VEGFR2 (A00901-2, Boster, Wuhan, China, 1 : 1000), p-Akt (#4691, Cell Signaling Technology, MA, United States, 1 : 1000), p-Akt (#4060, Cell Signaling Technology, MA, United States, 1 : 2000), VEGF 165a (ab69479, Abcam, Cambridge, MA, United States), VEGF 165b (GTX52502, GeneTex, Irvine, CA, United States), glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (A00227-4, Boster, Wuhan, China, 1 : 1000), at 4 °C overnight.

Techniques: Expressing, Activation Assay, Quantitative RT-PCR, Western Blot, Control

Journal: Biomedical Journal

Article Title: Inhibition of infantile hemangioma growth and promotion of apoptosis via VEGF/PI3K/Akt axis by 755-nm long-pulse alexandrite laser

doi: 10.1016/j.bj.2023.100675

Figure Lengend Snippet: Representative immunohistochemical staining of VEGFA, VEGFR2, PI3K, and Akt in tumor sections from each group of mice (magnification, × 400; scale bar, 25 μm). Abbreviations: VEGF: vascular endothelial growth factor; VEGFR: vascular endothelial growth factor receptor; PI3K: phosphoinositide 3-kinase; Akt: protein kinase B.

Article Snippet: The membranes were gently agitated and incubated with antibodies against the following proteins: VEGFA (BA0407, Boster, Wuhan, China, 1 : 1000), VEGFR2 (A00901-2, Boster, Wuhan, China, 1 : 1000), p-Akt (#4691, Cell Signaling Technology, MA, United States, 1 : 1000), p-Akt (#4060, Cell Signaling Technology, MA, United States, 1 : 2000), VEGF 165a (ab69479, Abcam, Cambridge, MA, United States), VEGF 165b (GTX52502, GeneTex, Irvine, CA, United States), glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (A00227-4, Boster, Wuhan, China, 1 : 1000), at 4 °C overnight.

Techniques: Immunohistochemical staining, Staining

Journal: Neuropathology : official journal of the Japanese Society of Neuropathology

Article Title: Co-existent pilocytic astrocytoma with acute B-cell leukemia within the cerebellum

doi: 10.1111/neup.12587

Figure Lengend Snippet: MRI (A) and histological (B, C) and immunohistochemical (D–H) findings of the tumor. A post-contrast enhancement T1-weighted image shows a cystic mass rim-enhancement in the right cerebellar hemisphere (A). Microphotographs of HE-stained sections demonstrate nests of tumor cells with ovoid nuclei and clear cytoplasm, and around the vascular septae is a condensation of small cells with a high nuclear:cytoplasm ratio (B, C). Immunohistochemistry for GFAP (D) and Olig2 (E) highlights the neoplastic glial components of the lesion. CD34 immunohistochemistry highlights perivascular lymphoblasts (F). VEGF immunoreactivity is localized in the glial components (G), while VEGFR-2 immunoreactivity is localized in the endothelial cells and a subset of lymphoblasts (H). Scale bars: 50 μm (B–H).

Article Snippet: For immunohistochemistry, we applied primary antibodies against Ki-67 (rabbit monoclonal, clone 30–9, prediluted; Ventana Medical Systems, Tucson, AZ, USA), glial fibrillary acid protein (GFAP) (rabbit monoclonal, clone EP672Y, prediluted; Ventana), oligodendrocyte transcription factor 2 (Olig-2) (mouse monoclonal, clone 211F1.1, prediluted; Sigma-Aldrich, St. Louis, MO, USA), CD34 (mouse monoclonal, clone QBEnd/10, prediluted; PA0354; Leica, Wetzlar, Germany), CD19 (mouse monoclonal, clone BT51E, prediluted; Leica), CD10 (mouse monoclonal, clone 5656, prediluted; Leica), VEGF-A (mouse monoclonal, clone JH121, diluted 1:25; Invitrogen, Waltham, MA, USA), and VEGF receptor 2 (VEGFR-2) (goat polyclonal, AF357, diluted 1:100; Bio-Techne, Minneapolis, MN, USA).

Techniques: Immunohistochemical staining, Staining, Immunohistochemistry